Characterization of Polyhydroxybutyrate Nanoparticles

Preparation of nanoparticles is one the important ways to increase biological effectiveness materials. There are several methods prepare polyhydroxybutyrate (PHB) nanoparticles. Here, a new method used based on exposing PHB ultrasound waves under variable pH conditions. In present study, was added distilled water and adjusted 4 by HCl (1 N). The suspension exposed at 4500 kh for 25 seconds. Then, readjusted 10 NaoH (1N) mixture incubated 2 h 21 oC. Finally, 7 N) oC 18 h. characterization prepared achieved using atomic force microscopy (AFM), Fourier-transform infrared spectroscopy (FTIR), ultraviolet (UV) spectrophotometer, X-ray powder diffraction (XRD), scanning electron (SEM). results demonstrated formation nanoparticles, especially after examinations SEM AFM, which showed that diameter particles between 22.9 73.95. study confirmed gradient high levels ultrasonic could produce